Lower prices for isotopicaly labelled mass-tag modifying reagent

Primetech launches lower prices for isotopicaly labelled mass-tag modifying reagent for metabolomic research - deuterated methyl chloroformate (d3-MCF).

 

 

# 0080

 

This molecule is useful for modification of polar metabolites in biological samples according to the protocol of Smart et al. (1) and can be used for global quantification in metabolomic reseach using GC-MS (2,3).

 

We also offer MCF (metabolomics grade). 

 

# 0081

 

Both compounds have the same reactivity in relation to organic acids, alcohols, amines, carbohydrates, and other polar metabolites but every D-MCF tag is 3 Da heavier compared to H-MCF modification to give good resolution in MS analysis.

 

(1) Smart KF, Aggio RB, Van Houtte JR, Villas-Bôas SG. Analytical platform for metabolome analysis of microbial cells using methyl chloroformate derivatization followed by gas chromatography-mass spectrometry. Nat Protoc. 2010 Sep;5(10):1709-1729.

 

http://www.ncbi.nlm.nih.gov/pubmed/20885382

 

(2) Kvitvang H-F-N, Andreassen T, Adam T, Villas-Boas S-G, Bruheim P (2011) Highly sensitive GC/MS/MS method for quantitation of amino and nonamino organic acids. Anal. Chem. 2011, 83, 2705–2711.

 

http://www.ncbi.nlm.nih.gov/pubmed/21388201

 

(3) S. Tumanov, V. Shmanai, D. Greenwood, Silas Villas-Boas. GC-(quadrupole)MS method for absolute quantification of polar metabolites in biological samples using deuterated methyl chloroformate as derivatization agent. Seventh International Conference of the Metabolomics Society. Cairns, Australia, 27-30 June 2011. 153.