Primetech extendes it's catalog of reagents for protein, peptides and other biomolecules labeling and launches the production of high quality reactive dyes used in Difference gel electrophoresis (DIGE).
We provide you to make a kit yourself.
The kit contains of four separate components in 5 nmol, 10 nmol, 25 nmol and bulk quantities:
|Product name||Cat. No.||Size/Scale||Price, €|
|By-2 NHS ester minimal dye||1417||5, 10, 25 nmol||55 €||Add to Cart|
|By-3 NHS ester minimal dye||140c||5, 10, 25 nmol||55 €||Add to Cart|
|By-5 NHS ester minimal dye||140d||5, 10, 25 nmol||55 €||Add to Cart|
|DMF (dimethylformamide), labeling grade||005f||10 ml||10 €||Add to Cart|
Primetech provides By-2 NHS ester minimal dye, By-3 NHS ester minimal dye, By-5 NHS ester minimal dye which are spectrally distinct, but mobility-matched. Thus, proteins labeled with these dyes co-migrate in gel-electrophoresis. Proteins are labeled with the above dyes via NHS ester chemistry. So, lysines are predominant sites of the labeling.
Best practice of 2D proteomics makes use of internal pooled control sample which is essentially a mixture of both proteomes labeled with one of the dyes. By-2 NHS ester minimal dye should be used for it. Other two dyes, By-3 NHS ester minimal dye and By-5 NHS ester minimal dye, are interchangeable for all experiments.
After the preparation of both protein mixtures being compared, the following protocol can be used to achieve labeling.
You may simly e-mail us to order your kit, just specify the quontity.