Cyanovinylcarbazole phosphoramidite (CNVK) - NEW PRODUCT

Primetech now offers 3-cyanovinylcarbazole (^CNVK). Oligonucleotides containing 3-cyanovinylcarbazole (^CNVK) can be rapidly cross-linked to a complementary strand at UV 366nm without causing significant DNA damage.^1-3 In addition, studies have shown that purine bases were unreactive to cross-linking, allowing differentiation between pyrimidines and purines at the target site. Once cross-linked, the UV melting temperature of the duplex was raised by around 30°C (relative to the duplex temperature before irradiation. Complete reversal of the cross-link takes place at 312nm in approximately three minutes. 

References:

1.   (1) Y. Yoshimura, and K. Fujimoto, Org Lett, 2008, 10, 3227-30.
      (2) K. Fujimoto, K. Konishi-Hiratsuka, T. Sakamoto, and Y. Yoshimura, ChemBioChem, 2010, 11, 1661-4.
      (3) Y. Yoshimura, T. Ohtake, H. Okada, and K. Fujimoto, ChemBioChem, 2009, 10, 1473-6