Stearyl Phosphoramidite

Stearyl Phosphoramidite

Cat. number: 1135
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In Stock
Price: on request

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Description

Synthetic oligonucleotides often require lipophilic and non-toxic modification to carry them into desired cellular targets. The straightforward way to achieve such a modification is the automatic 5'-coupling with an appropriate lipid phosphoramidite. Several publications have shown that this approach is useful for the silencing of gene expression with siRNAs in vivo (Nat. Biotech. 2007, 25, 1149–1157, doi: 10.1038/nbt1339).

We now offer an affordable C18 lipid modifier which is easy to use under the standard synthesis conditions. The obtained 5'-stearyl oligonucleotides can be purified using RP cartridges or standard chromatographic techniques.

Catalog Number

1135

Molecular Formula                    

C27H55N2O2P

Molecular Weight

470.71 g/mol

Chemical Name

Octadecyl-1-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Appearance

Colorless oil

Assay

93 % (NMR 31P)

Storage Conditions

Keep in a dry place at -20°C

Shelf Life

1 year

Usage

Dilution: dilute 100 mg of stearyl phosphoramidite in 2.12 mL of anhydrous acetonitrile/dichloromethane 1:3 (v/v) to obtain 0.1 M solution

Oxidation: standard protocol

Coupling: 3 minute coupling time is recommended

Deprotection: standard protocol

1H NMR (CD3CN)

Conforms to the internal standard

31P NMR (CD3CN)

Conforms to the internal standard

 

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