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Stearyl phosphoramidite
Stearyl phosphoramidite
Description
Synthetic oligonucleotides often require lipophilic and non-toxic modification to carry them into desired cellular targets. The straightforward way to achieve such a modification is the automatic 5'-coupling with an appropriate lipid phosphoramidite. Several publications have shown that this approach is useful for the silencing of gene expression with siRNAs in vivo (Nat. Biotech. 2007, 25, 1149–1157, doi: 10.1038/nbt1339).
We now offer an affordable C18 lipid modifier which is easy to use under the standard synthesis conditions. The obtained 5'-stearyl oligonucleotides can be purified using RP cartridges or standard chromatographic techniques.
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Catalog Number |
1135 |
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Molecular Formula |
C27H55N2O2P |
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Molecular Weight |
470.71 g/mol |
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Chemical Name |
Octadecyl-1-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite |
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Appearance |
Colorless oil |
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Assay |
93 % (NMR 31P) |
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Storage Conditions |
Keep in a dry place at -20°C |
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Usage |
Dilution: dilute 100 mg of stearyl phosphoramidite in 2.12 mL of anhydrous acetonitrile/dichloromethane 1:3 (v/v) to obtain 0.1 M solution Oxidation: standard protocol Coupling: 3 minute coupling time is recommended Deprotection: standard protocol |
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1H NMR (CD3CN) |
Conforms to the internal standard |
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31P NMR (CD3CN) |
Conforms to the internal standard |
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