CAS Number

-

Molecular Formula

C₂₅H₃₈N₃O₄PS

Molecular Weight

507,63 g/mol

Mass Contribution

265,05 g/mol

Chemical Name

S-(3-(((1r,4r)-4-(((2-cyanoethoxy)(diisopropylamino)phosphaneyl)oxy)cyclohexyl)amino)-3-oxopropyl) benzothioate 2-cyanoethyl N,N-diisopropylphosphoramidite

Appearance

Pale yellow oil

Assay

>98% (³¹P NMR)

Storage Conditions

Keep under argon atmosphere in a dark and dry place at -20°C

Usage

Activator for synthesis: 5-Benzylthio-1H-tetrazol (BTT) recommended.

Coupling: 3 minute coupling time recommended.

Deprotection: Synthesize using acetyl-protected dC. Prior to cleavage, treat support with 10% diethylamine in acetonitrile for 5 minutes at room temperature and rinse with acetonitrile to remove the cyanoethyl protecting groups. Then cleave and deprotect the oligo using 30% ammonium hydroxide/40% methylamine 1:1 (AMA) for 2 hours at room temperature. Prior to use, reduce any disulfide formation using 100 mM
tris(2-carboxyethyl)phosphine (TCEP) or dithiothreitol (DTT) for 30 minutes at room temperature.

¹H NMR (CD₃CN)

Conforms to the internal standard

³¹P NMR (CD₃CN)

Conforms to the internal standard