Molecular Formula

C₇₂H₈₃N₄O₁₄P

Molecular Weight

1259.4 g/mol

Chemical Name

5-((6-((2S,4R)-2-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-4-(((2-cyanoethoxy)(diisopropylamino)phosphanyl)oxy)pyrrolidin-1-yl)-6-oxohexyl)carbamoyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl bis(2,2-dimethylpropanoate)

Appearance

White powder

Assay

≥99% (NMR)

Storage Conditions

Keep in a dark and dry place at -20°C

Usage

Dilution: dilute 100 mg of 6-FAM phosphoramidite Pro in 0.8 mL of anhydrous acetonitrile to obtain 0.1M solution

Oxidation: standard conditions are recommended

Coupling: 5 minute coupling time is recommended

Deprotection: 60°С overnight in conc. ammonia is recommended

¹H NMR (CD₃CN)

Conforms to the internal standard

Contains traces of toluene

³¹P NMR (CD₃CN)

Conforms to the internal standard