Molecular Formula

C₄₆H₅₈N₃O₁₀P

Molecular Weight

843.9 g/mol

Mass contribution

552,5 g/mol

Chemical Name

5-((6-(((2-cyanoethoxy)(diisopropylamino)phosphino)oxy)hexyl)carbamoyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl bis(2,2-dimethylpropanoate)

Appearance

White powder

Assay

≥98% (NMR)

Storage Conditions

Keep in a dark and dry place at -20°C

Usage

Dilution: dilute 100 mg of 5-FAM phosphoramidite in 1.2 mL of anhydrous acetonitrile to obtain 0.1M solution

Oxidation: standard conditions are recommended

Coupling: 5 minute coupling time is recommended

Deprotection: 60°С overnight in conc. ammonia is recommended

¹H NMR (CD₃CN)

Conforms to the internal standard

³¹P NMR (CD₃CN)

Conforms to the internal standard