By-3 phosphoramidite

By-3 phosphoramidite

Cat. number: 1120
Availability:
In Stock
Price: 80 €

Available Options


Description

Molecular Formula

C45H65BF4N5O3P

Molecular Weight

841.8 g/mol

Chemical Name

3,3-dimethyl-1-(5-{[(1R,4R)-4-({[bis(propan-2-yl)amino](2-cyanoethoxy)phosphanyl}oxy)cyclohexyl]carbamoyl}pentyl)-2-[3-[1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium tetrafluoroborate

Appearance

Metallic green or red powder

Assay

≥94% (31P NMR)

Storage Conditions

Keep in a dark and dry place at -20°C

1H NMR (CD3CN)

Conforms to the internal standard

31P NMR (CD3CN)

Conforms to the internal standard

Coupling test (with T10

oligonucleotide)

Passed

Shelf Life

1 year

Usage

Dilution: dilute 100 mg of By-3 phosphoramidite in 1.2 mL of anhydrous acetonitrile to obtain 0.1M solution

Oxidation: 0.02 M iodine is recommended

Coupling: 3 minute coupling time is recommended

Deprotection: 24 hours at room temperature in conc. ammonia is recommended