Molecular Formula

C₄₅H₆₅BF₄N₅O₃P

Molecular Weight

841.8 g/mol

Chemical Name

3,3-dimethyl-1-(5-{[(1R,4R)-4-({[bis(propan-2-yl)amino](2-cyanoethoxy)phosphanyl}oxy)cyclohexyl]carbamoyl}pentyl)-2-[3-[1,3,3-trimethyl-2,3-dihydro-1H-indol-2-ylidene]prop-1-en-1-yl]-3H-indol-1-ium tetrafluoroborate

Appearance

Metallic green or red powder

Assay

≥94% (³¹P NMR)

Storage Conditions

Keep in a dark and dry place at -20°C

¹H NMR (CD₃CN)

Conforms to the internal standard

³¹P NMR (CD₃CN)

Conforms to the internal standard

Coupling test (with T₁₀

oligonucleotide)

Passed

Shelf Life

1 year

Usage

Dilution: dilute 100 mg of By-3 phosphoramidite in 1.2 mL of anhydrous acetonitrile to obtain 0.1M solution

Oxidation: 0.02 M iodine is recommended

Coupling: 3 minute coupling time is recommended

Deprotection: 24 hours at room temperature in conc. ammonia is recommended. By 3 is compatible with UltraFast deprotection (AMA 10 minutes at 65 °C) if Ac-dC was used.