CAS Number

1644576-49-7

Molecular Formula

C₄₂H₅₅F₃N₅O₇P

Molecular Weight

828.9 g/mol

MALDI Mass Contribution

292.1 (plus to non-modified oligonucleotide mass)

Chemical Name

(3R,5S)-5-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-1-(6-(2,2,2-trifluoroacetamido)hexanoyl)pyrrolidin-3-yl(2-cyanoethyl)diisopropyl phosphoramidite

Appearance

White foam

Assay

≥95% (³¹P NMR)

Storage Conditions

Keep in a dark and dry place at -20°C. Store under argon

Usage

Dilution: dilute 100 mg of Amino-modifier Pro phosphoramidite in
1.21 mL of anhydrous acetonitrile to obtain 0.1M solution

Oxidation: standard iodine solution is recommended

Coupling: 5 minutes coupling time is recommended

Deprotection: 60°C overnight in conc. ammonia is recommended

¹H NMR (CD₃CN)

Conforms to the internal standard

³¹P NMR (CD₃CN)

Conforms to the internal standard