MitoLumina 640 is a cell-permeant carbocyanine fluorescent dye specifically designed for labeling active mitochondria in live cells. It contains a mildly thiol-reactive chloromethyl group that allows it to covalently bind to mitochondrial proteins, ensuring retention even after cell fixation and permeabilization.

With excitation and emission maxima at approximately 640 and 662 nm respectively, it emits in the far red spectrum, making it ideal for multicolor fluorescence imaging alongside other probes.

MitoLumina 640 is a close analog of MitoTracker Deep Red FM, which is widely applied in cell biology, neuroscience, and cancer research to visualize mitochondrial morphology, distribution, and dynamics.

Mitochondria imaging with MitoLumina 640 in fibroblast cells