MitoLumina 545 is a cell-permeant carbocyanine fluorescent dye specifically designed for labeling active mitochondria in live cells. It contains a mildly thiol-reactive chloromethyl group that allows it to covalently bind to mitochondrial proteins, ensuring retention even after cell fixation and permeabilization.

With excitation and emission maxima at approximately 545 and 568 nm respectively, it emits in the red spectrum, making it ideal for multicolor fluorescence imaging alongside other probes.

MitoLumina 545 belongs to the same class of mitochondria stains as MitoTracker Deep Red FM but has slightly shifted excitation and emission maxima. It can be applied in cell biology, neuroscience, and cancer research to visualize mitochondrial morphology, distribution, and dynamics.

Mitochondria imaging with MitoLumina 545 in fibroblast cells