Dual-labelled probes for real-time qPCR

Oligonucleotides order form

Double-Dye Oligonucleotides (or dual-labeled probes) are the most widely used type of probes in real-time qPCR.

Comparing to non sequence-specific ZUBR Green-1 detection format dual-labeled probes provide extremely high sensitivity and specificity.

Primetech offers you different formats of dual-labeled probes:

  • - TaqMan probes
  • - Molecular Beacons
  • - Scorpions
  • - Amplifluor UniPrimers, etc.

Each dual-labeled probe can be synthesized with a wide range of fluorophores and quenchers in various scales.

Prices for dual-labeled probes include:

  • - base addition
  • - dye labeling
  • - three-step purification.

 

Probe Price*, €
1 OD260 3 OD260 5 OD260 10 OD260
  • FAM - Dabcyl/BHQ-1
  • R110 - BHQ-1
  • TET - BHQ-1
  • JOE - BHQ-1/BHQ-2
  • R6G - BHQ-1/BHQ-2
  • HEX - BHQ-1/BHQ-2
  • TAMRA - BHQ-1/BHQ-2
  • ROX - BHQ-2
  • By-3 - BHQ-2
  • By-3.5 - BHQ-2
  • By-5 - BHQ-2
  • By-5.5 - BHQ-2
70 100 120 200

LUX probe (FAM)

55 70 80 100
LUX probe (JOE) 70 100 120

130

 Wavelenghts of commonly used dyes and quenchers