5’-phosphorylation phosphoramidite

5’-phosphorylation phosphoramidite

Cat. number: 111d
Availability:
In Stock
Price: 45 €

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Description

Product Name

5’-phosphorylation phosphoramidite

Catalog Number

111d

CAS Number

108783-02-4

Molecular Formula

C34H45N2O7PS

Molecular Weight

656.3 g/mol

MALDI Mass Contribution

80.0 (plus to non-modified oligonucleotide mass)

Chemical Name

2-((2-((4,4’-dimethoxytrityl)oxy)ethyl)sulphonyl)ethyl(2-cyanoethyl)diisopropylphosphoramidite

Appearance

Yellowish oil

Assay

≥95% (31P NMR)

Storage Conditions

Keep in a dark and dry place at -20°C. Store under argon

Shelf Life

1 year

Usage

Dilution: dilute 100 mg of Alkylacetylene phosphoramidite Pro in
1.52 mL of anhydrous acetonitrile to obtain 0.1M solution

Oxidation: standard iodine solution is recommended

Coupling: 5 minutes coupling time is recommended

Deprotection: 60°C overnight in conc. ammonia is recommended

1H NMR (CD3CN)

Conforms to the internal standard

31P NMR (CD3CN)

Conforms to the internal standard